Quantitative comparison of the storage protein distribution in glandless and glanded cottonseeds

Glanded (Gd) cottonseed (Gossypium hirsutum L.) contains scattered gossypol glands. Glandless (Gl) is a new type of seed containing only trace levels gossypol. This work quantitatively compared the content and migration pattern Gd Gl protein isolates. Both samples were subjected to sodium dodecyl sulfate (SDS)-gel electrophoresis, gel bands separated into seven partitions for peptide mass spectroscopic analysis. While multiple fragments (isoformers) vicilin legumin proteins present in both samples, percentage vicilins total was higher (74.9%) than (63.4%). In contrast, more abundant (30.4%) (23.6%). Minor components such as lipid-related oleosins vicilin-like antimicrobial peptides 2-2 also observed at relatively incidence with Gd, potentially reflecting need increased protein-related defense capability absence against natural predators or adverse growth environment.